Innovative Perspectives of Integrated Chinese Medicine on H. pylori / 中国结合医学杂志

Helicobacter pylori (H. pylori) treatment requires the development of more effective therapies, mainly owing to the challenges posed by the bacterial resistance to antibiotics. In China, critically high infection and antibiotic resistance rates have limited the application of classic H. pylori eradication therapies. Consequently, researchers are attempting to find new solutions by drawing from traditional medicine. This article reviews basic scientific and clinical progress in the use of integrated Chinese and Western medicine (IM) to treat H. pylori; describes the conflicting results between in vivo and in vitro studies in this regard; discusses the observed clinical effects of IM, with emphasis on traditional patent medicines; and proposes a role for IM in both the diagnosis and treatment of H. pylori, including the use of tongue manifestation as an early diagnostic method and capitalizing on IM's direct and indirect methods for enhancing antibiotic effect.

Anti-bacterial and anti-inflammatory effects of Qingre Huashi Formula on Helicobacter pylori-infected mice / 中成药

AIM To evaluate the bactericidal activity of Qingre Huashi Formula (Coptidis Rhizoma,Gardeniae Fructus,Taraxaci Herba,etc.) against Helicobacter pylori in infected mice and the effects on NF-κB p65,IL-1 β,IL-8 and TNF-α expression levels.METHODS Forty-four SPF KM mice were randomly divided into four groups,blank control,model control,Qingre Huashi Formula and triple therapy (metronidazole,clarithromycin and lansoprazole) groups.Except the blank control group,mice in another three groups were infected with H.pylori through short-term immunosuppression combined with oral infection.Administration lasted for four weeks.All mice were sacrificed.Rapid urease test (RUT) and histological staining were performed to identify the eradication of H.pylori.HE staining was applied to observing the H.pylori colonization degree and gastric mucosal inflammation.NF-κB p65,IL-1 β,IL-8 and TNF-α expression levels in gastric mucosa were measured through fluorescentquantitative PCR and immunohistochemistry.RESULTS H.pylori eradication rates were model control 0/10,Qingre Huashi Formula 3/10,triple therapy 7/10.Under the light microscope observation,model control group possessed massive H.pylori colonization and partial inflammatory cell infiltration.In triple therapy group,H.pylori colonization and inflammation was slight.Qingre Huashi Formula group had much local accumulation of H.pylori with no obvious inflammatory cell infiltration.The Il1b,Il8,Tnfa and Nfkb p65 mRNA expression levels of triple therapy and Qingre Huashi Formula groups were significantly higher than those of model control group.Immunohistochemistry results displayed no statistical significance among triple therapy,model control and Qingre Huashi Formula groups in the ratio of nucleus/cytoplasm optical density.CONCLUSION Qingre Huashi Formula has a limited eradication activity against H.pylori in infected mice with an obvious improvement of gastric mucosa inflammation.The Ill b,Il8,Tnfa and Nfkb p65 mRNA expression levels of Qingre Huashi Formula and triple therapy groups' markedly increasing with similar expression tendency is possibly related to the negative feedback effect induced by inhibition of protein levels,thus affects the occurance of inflammatory.

In vitro bactericidal activity of Jinghua Weikang Capsule and its individual herb Chenopodium ambrosioides L. against antibiotic-resistant Helicobacter pylori / 中国结合医学杂志

<p><b>OBJECTIVE</b>To investigate the bactericidal effects of Jinghua Weikang Capsule and its major component Chenopodium ambrosioides L. on antibiotic-resistant Helicobacter pylori.</p><p><b>METHODS</b>Four clinical antibiotic-resistant H. pylori strains were isolated and incubated in liquid medium containing Jinghua Weikang Capsule or Chenopodium ambrosioides L. By means of time-kill curve method, the average colony counts and bactericidal rate were calculated at time points of 0, 4, 8 and 24 h after the incubation and the time-kill curves were charted.</p><p><b>RESULTS</b>Both Jinghua Weikang Capsule and Chenopodium ambrosioides L. at a concentration of 0.64 g/L showed obvious bactericidal effect against antibiotic-resistant H. pylori after 4 h of incubation.</p><p><b>CONCLUSION</b>Jinghua Weikang Capsule and Chenopodium ambrosioides L. are considered to be active against antibiotic-resistant H. pylori in vitro.</p>

Involvement of sympathetic nervous system in the pathogenesis of hypertension / 中华医学遗传学杂志

Sustained activation of sympathetic nervous system in response to stimulation of a wide variety of stress factors is an independent risk factor for the development of essential hypertension. Adrenal hormone biosynthesis pathway as an important part of the sympathetic nervous system consists of hormones, neurotransmitters, receptors, and a variety of synthases and invertases. In this article, we have systematically reviewed research progresses made in elucidating the interactions between genes of the adrenal hormone biosynthesis pathway and stress factors in the pathogenesis of essential hypertension.

Treatment of non-alcoholic fatty liver disease by Qianggan Capsule / 中国结合医学杂志

<p><b>OBJECTIVE</b>To observe the therapeutic effect and safety of Qianggan Capsule (QGC) in treating non-alcoholic fatty liver disease (NAFLD), using polyene phosphatidylcholine capsule (PPC) as a reference.</p><p><b>METHODS</b>Eighty-eight patients with NAFLD were randomly assigned to two groups, 45 in the treatment group treated with QGC and 43 in the control group treated with PPC. The course of treatment lasted for 6 months. Changes in liver function, blood lipids, and iconographic indexes before and after treatment were observed, and clinical efficacy was evaluated.</p><p><b>RESULTS</b>In the treatment group, alanine aminotransferase (ALT) was lowered significantly from 56.02 + or - 32.59 IU/L before treatment to 38.27 + or - 22.68 IU/L after treatment, and CT liver/spleen ratio significantly increased from 0.69 + or - 0.18 to 0.91 + or - 0.25, showing statistical significance (P<0.05); in contrast, the corresponding changes of the two indexes in the control group were 56.56 + or - 26.33 IU/L to 49.67 + or - 26.22 IU/L, and 0.66 + or - 0.20 to 0.75 + or - 0.24, respectively, the pre-post treatment difference showing insignificant difference (P>0.05). No severe adverse reactions occurred during the whole treatment course.</p><p><b>CONCLUSION</b>QGC is an effective and safe remedy for the treatment of NAFLD.</p>